It takes considerable practice to master the art of "running a column". This video will get you started, with tips on picking appropriate conditions, packing and running a column, monitoring separation, and even making a micro-column from a pipet.
Created by Dr. Sarah Tabacco and Aaeyesha Siddiqui
View the complete resource at: http://ocw.mit.edu/OcwWeb/resources/RES-5-0001Spring-2007/ResourceHome
License: Creative Commons BY-NC-SA
More information at http://ocw.mit.edu/terms
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The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented. Legal Notice - http://ocw.mit.edu/OcwWeb/web/terms/terms/index.htm
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