It takes considerable practice to master the art of "running a column". This video will get you started, with tips on picking appropriate conditions, packing and running a column, monitoring separation, and even making a micro-column from a pipet.
View the complete course: http://ocw.mit.edu/5-301IAP04
License: Creative Commons BY-NC-SA
More information at http://ocw.mit.edu/terms
More courses at http://ocw.mit.edu
Tagged under: mit,opencourseware,chemistry,laboratory,purification,extraction,transfer,chromatography,experiment,spectraoscopy
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